★ Master mix (PCR)
A master mix is a mixture containing precursors and enzymes used as an ingredient in RT-PCR methods in molecular biology. Such mixtures contain a mixture of dNTPs, MgCl 2, Taq-polymerase, pH-buffer and enter mixed into nuclease-free water.
Master mixtures for real-time PCR include a fluorescent compound, often SYBR green, and the choice of mixture also affects the sensitivity of the test and the consistency.
Differences in the choice of basic mixtures can sometimes explain differences in experimental results, a special case of which is the measurement of telomere length.
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